Lithium Carbonate as A Bluing Agent – A Comparative Study
*Corresponding Author E-mail: firstname.lastname@example.org
Bluing is the process which changes the initial soluble reddish-purple Hematoxylin into an insoluble darker bluish-purple. After differentiation with acid, the tissue sections are subsequently treated with an alkaline solution or vapours to neutralize the acid and restore the blue colour to the tissue. Commonly used bluing agents in histopathology are tap water, Scott's tap water and Ammonium Hydroxide water. The aim of this study is to assess the efficacy of 0.05% aqueous solution of Lithium Carbonate as an alternative to Ammonium hydroxide water as a bluing agent.
Materials and Methods
0.05% aqueous solution of Lithium Carbonate was freshly prepared by dissolving 0.05g of Lithium Carbonate powder in 100ml of Distilled Water. Ammonium Hydroxide Water was prepared by adding 5ml of Ammonium hydroxide solution to 100 ml of distilled water. From each of the five blocks procured, two sections were cut using soft tissue microtome. After staining with Haematoxylin, the five test group slides were immersed in 0.05% aqueous solution of Lithium carbonate, while the five control group slides were immersed in ammonium hydroxide water for bluing. After counterstaining with Eosin and dehydration with graded alcohol, the slides were mounted and rated by two blinded observers.
The nuclear and cytoplasmic staining intensity, Bluing and clarity was better when 0.05% aqueous solution of Lithium carbonate was used as a bluing agent.
0.05% aqueous solution of lithium carbonate can be used as an alternative to Ammonium hydroxide water for bluing.
Bluing, Ammonium hydroxide water, Lithium carbonate, Haematoxylin and Eosin staining, Histopathology.