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Research Journal of Pharmacy and Technology
Year : 2019, Volume : 12, Issue : 1
First page : ( 297) Last page : ( 302)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2019.00055.6

Isolation and Characterization of Phytochemical Constituents and its Antibacterial Activity of Brassica oleracea var acephala

Rao U. S. Mahadeva1, Sundaram C. Shanmuga2,*, Sivakumar J.3

1Faculty of Medicine, Universiti Sultan Zainal Abidin, 20400, Terengganu, Malaysia

2PG & Research, Department of Microbiology, Hindustan College of Arts & Science, Padur, Chennai-603103

3PG & Research Department of Biotechnology, Hindustan College of Arts & Science, Padur, Chennai-603103

*Corresponding Author E-mail: amudhashanmugam1977@gmail.com

Online published on 16 March, 2019.



Traditional medicines are mostly depends on medicinal plants. These are considered as good resources of ingredients which can be used in drug formulation and combination. Hence the present study is to investigate the valuable pharmacological activities ofBrassica oleracea var acephala.

Materials and Methods

The collected fresh foliage were washed, dried, chopped and powdered for the preparation of ethanolic crude extract. By using this ethanolic crude extract of Brassica oleracea var acephala phytochemical analysis were done. The crude extract was tested against various bacterial strains and fungal strains by Agar Well diffusion assay. Then the crude extract was subjected to GC-MS and FTIR analysis for the identification of bioactive compounds.


Preliminary phytochemical test showed the presence of Phenolic compounds, Tannins, Terpenoids, Phytosterol and Flavonoids. The results of the antibacterial screening of the crude extract on the S.aureus showed more inhibition. The ethanolic crude extract showed that eleven compounds were identified through GC-MS and FTIR analysis.


Brassica oleracea var acephala has been studied and the results of the present investigation revealed that the leaf extract contained high phytochemical activity. The present study attempted to establish a relationship between ethnopharmacological claims and bioactive constituents present in Brassica oleracea var Acephala against all possible targets for cancer.



Brassica oleracea var acephala, phytochemical screening, antibacterialactivities, GC-MS analysis, FTIR analysis.


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