Biochemical Characterization of Anti-Microbial Activity and Purification of Glycolipids Produced by Dodecanoic Acid-Undecyl Ester
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Microorganisms displaying antimicrobial action were disengaged from oil defiled destinations in Tadepalli, Andhrapradesh, India. It was described and distinguished as Dodecanoic acid undecyl ester. The biosurfactants created by Dodecanoic acid undecyl ester were refined and portrayed by TLC, GC, HPLC and GC-MS. The impact of antimicrobial exercises were acquired by utilizing agar dispersion strategy against some Gram positive and Gram negative pathogenic microorganisms included (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Proteus spp., Corynebacterium spp., Salmonella spp., Streptococcus pneumonia, Pseudomonas aerouginosa and Bacillus cereus); and two pathogenic organisms viz., Aspergillus niger and Aspergillus flavus. These surface dynamic operators showed high inhibitory action against Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Aspergillus flavus among the tried ones. The outcomes plainly showed that the antibacterial and antifungal movement of the utilized glycolipids change with the types of the living beings utilized. The microorganisms inspected by filtering electronic magnifying instrument (SEM), were completely twisted and displayed extreme decimation. Irregular cell division was seen at high frequencies among cells that attempted to isolate within the sight of the Dodecanoic acid undecyl ester glycolipids. Numerous cells were expanded, stretched, discharge has, or divided, reliable with the amazingly low feasibility. Hence, the examination finds out the estimation of the utilization of these glycolipids which could be of extensive enthusiasm to the advancement of new hostile to microbial materials for restorative applications as water cleansing plants, dental surgery types of gear, oil recuperation and creation, overwhelming metal expulsion from water amid oil refinery process and pharmaceutical purposes. The potential movement of these microorganisms to create valuable antimicrobial mixes is incredible and must be better investigated.
Biosurfactants, Purification, Antimicrobial Activity, TLC, HPLC, GC-MS, Glycolipid.