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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 9
First page : ( 3905) Last page : ( 3908)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00716.3

Designing and Development of Rice water based crude media and it's application in fungal isolation and enzyme production

Thiruvengadam S., Jeevanantham S., Kamalesh R.*, Hamsini S, Kamali T. B, Karishma S., Jayalakshmi H.

Department of Biotechnology, Rajalakshmi Engineering College, Chennai-602105

*Corresponding Author E-mail: kamaleshsuriya1998@gmail.com

Online published on 20 December, 2018.

Abstract

Crude media is generally used on an industrial scale for microbial cultivation, inoculums preparation and fermentation process. Crude media contains a rough composition of media required for microbial propagation. It contains high level of undefined sources of nutritional ingredients like Carbohydrates, proteins, vitamins, growth factors, anti-foaming agents and precursors. Aim of our current research work focused to design rice water based crude medium for microbial isolation and cultivation from soil. Fertile soil sample was collected from garden and allowed to dilution in distilled water. Then the diluted sample were inoculated with 4% of rice water and incubated in room temperature for a week. In order to avoid bacterial contamination antibacterial antibiotic was incorporated. well developed and distinguished fungal colonies were sub cultured in different rice water composition and allowed for incubation. The isolated fungal strains were studied by LCB wet mount preparations and identified as Mucor, Rhizopus and Aspergillus species. In this study, it is reported that the composition of 4% rice gives effective growth specifically Mucor, Rhizopus and Aspergillus species. As it is economically cheap it can be used for the microbial fermentation in industrial level.

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Keywords

Rice water, Crude medium, Fermentation, MucorRhizopusAspergillus..

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