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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 9
First page : ( 3777) Last page : ( 3780)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00692.3

Prevalence of extended spectrum ß-Lactamase and Carbapenemase producing isolates of Klebsiella SPP in a tertiary care hospital

Syntem Lead Me On1, Dutta Himadri2,*, Neelusree P3, Kalyani M4

1Department of Microbiology, ACS Medical College and Hospital

2Assistant Professor, Department of Microbiology, Saveetha Medical College and Hospital

3Associate Professor, Department of Microbiology, Saveetha Medical College and Hospital

4Professor and Head, Department of Microbiology, Saveetha Medical College and Hospital

*Corresponding Author E-mail: drhimadrik@gmail.com

Online published on 20 December, 2018.

Abstract

Objectives

Wide spread use of β lactam antibiotics has lead to resistance to these group of antibiotics in bacterial pathogens due to various types of β lactamase production. Klebsiella pneumoniae harboring Extended spectrum β lactamase (ESBL)) and carbapenemases are a serious problem worldwide. Klebsiella pneumoniae is an established pathogen responsible for multidrug resistant hospital acquired infections. Our study was undertaken to detect prevalence of ESBL and KPC producing Klebsiella spp in a tertiary care hospital and to check their antibiotic resistance pattern by phenotypic methods recommended by CLSI.

Methods

A total of 120 non enteric clinical isolates klebsiella spp were speciated and antibiotic sensitivity was determined by Kirby Bauer disc diffusion method. Isolates found to be screen positive for possible ESBL and carbapenemase production were further confirmed by phenotypic confirmatory methods recommended by CLSI.

Results

I09 isolates were found to be screen positive for ESBL, out of which 29 isolates (27%) were confrmed ESBL producer by combined disc method. A total of 11 isolates were found to be screen positive for carbapenemase production out of which 4 (36%) were confirmed carbapenemase (KPC) producer by Modified Hodge test (MHT). All the ESBL and carbapenemase producing Klebsiella isolates were found to be multidrug resistant (MDR). 93% (27/29) ESBL positive isolates were sensitive to imipenem. ESBL producers also showed coresistance to amikacin 52% (15/29), ciprofloxacin 41% (12/29). Two carbapenemase producing 2 isolates were highly resistant and found to be sensitive to only colistin and tigecycline.

Conclusion

Failure to detect MDR organisms like ESBL and KPC producers on time may lead to outbreak of hospital acquired infections by such strains. So clinical laboratories should routinely do such easy to perform screening tests to detect these resistance mechanisms.

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Keywords

Multidrug resistance, ESBL, Carbapenemase, nosocomial, KPC.

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