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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 8
First page : ( 3641) Last page : ( 3646)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00671.6

Ecological Niche Analysis of Streptomyces sp. Amet_ytc 21 nov. Strain Isolated from Marine Intertidal Zones of bay of Bengal and Buckingham Canal

Chelvan Yashika1,2,*, Chelvan Thamil2,3, Vanitha M. C.2

1Nehru Foundation for Development, Vikram A Sarabhai Community Science Centre (VASCSC), Opp. Gujarat University, Navrangpura, Ahmedabad, Gujarat-380 009

2Department of Marine Biotechnology, AMET University, Kanathur, Chennai, 603112, Tamil Nadu, India

3Department of Bioscience, Narmada College of Science and Commerce, Shukltirth Road, Zadeshwar, Bharuch, Gujarat-392011

*Corresponding Author E-mail: yashikachelvan@gmail.com

Online published on 31 October, 2018.

Abstract

Objective

To explore intertidal zone Swell seawater (High tide) for presence of novel Halotolerant Marine Actinomycetes (HTMA).

Methods

Sea water analysis performed to estimate physiochemical parameters of unique Habitat. Phenotypic and genotypic analysis of isolated Halotolerant Marine Actinomycetes strains. Bioinformatics based analysis of novel HTMA Strain AMET YTC_21.

Results

Physiochemical parameters of samples were as Temperature Seawater (28.00±0.50)°C, Salinity (34.96±0.05) ppt, Electrical Conductivity (34.96±0.05) μs/cm, pH (8.88±0.084), Dissolved Oxygen (11.94±0.11) mg/L, NO3 (0.25±0.021) mg/L, Total Dissolved Solids (27850.2±0.44) mg/L and Total PO4(0.013±0.00)mg/L. Salt tolerance, pH and temperature tolerance of selected HTMA strains and selection of potential strain for genetic analysis. Bioinformatics based Homology modeling and Phylogenetic tree analysis of novel Streptomyces sp. AMET_YTC21 strain.

Conclusion

In the present study molecular identification by 16s rRNA gene sequencing of the isolated HTMA strain confirmed the organism a streptomyces sp. AMET_YTC21. The 16s rRNA nucleotide sequence was submitted to Gen bank in NCBI and the accession number obtained as Bank It KY072795. Morphological and biochemical evidence also suggested the presence of novel strain with high pH, Salt and temperature tolerance as Halotolerant Marine Actinomycetes. The obtained results will help us to determine the right candidate for novel therapeutic enzyme production.

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Keywords

Bay of Bengal, Brackish water, Halotolerant Marine Actinomycetes, Bioinformatics.

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