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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 4
First page : ( 1505) Last page : ( 1512)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00280.9

Impact of Human Cytomegalovirus Infection associated with the expressed protein of mutated BRCA1 gene in breast tissues from a group of Iraqi Female Patients with Breast Carcinoma

Ali Saad H. Mohammed1,*, Almahbobi Taghreed F2, AL-Bayaa Yasmeen J.1, Al-Alwany Shakir H. Mohammed3

1College of Medicine, Baghdad University, Iraq

2College of Medicine, Jaber Ibn Hayyan Medical University, Iraq

3College of Science, Babylon University, Iraq

*Corresponding Author E-mail: xaviersuresh@gmail.com

Online published on 24 July, 2018.

Abstract

It has been reported that Cytomegalovirus causes breast cancer in mice while human cytomegalovirus might have role in breast cancer progression and activation of protein kinase.BRCA1 and BRCA2 proteins help both to repair damaged DNA and ensure cellular genomic stability and cells with specific inheritedmutations or alterations in these genes are more likely for genetic alterations and cancer development, as in female breast and ovarian cancers as well as several other cancers. The present study aims to analyze both the rates of HCMV infection as well as defects and/or mutations in BRCA-1 gene in a group of primary invasive breast cancer tissues and to assess their relations to the differentiation of these cancers. about Seventy breast tissues were enrolled in this study; (30) breast cancers, (25) from benign breast tumors and (15) apparently healthy breast tissues as control groups. Detection of HCMV was done by ultra-sensitive version of in situ hybridization method whereas immunohistochemistry detection system was used to demonstrate the expression of BRCA1 gene. The Results show that the HCMV DNA-ISH reactions in breast cancertissues was observed in 17 out of 30 (56.7%), while in in benign breast tumor tissues was detected in 24% (6 out of 25). Nopositive ISH reactions of HCMV DNA were detected in control breast tissues group. The differences between the percentages of HCMV detection in breast cancer tissues and each of benign breast tumor & control groups were statistically highly significant (P value = < 0.0001). Positive BRCA1 immunohistochemical reactions were observed in 16 breast cancer tissues (53.3%) and in 11 benign breast tumor tissues (44%). However, statistical significant differences between the frequencies of HCMV and BRCA-1-immunohistochemical reactions were neither observed in relation to the age of these breast cancer patients nor to the grade of invasive breast cancer tissues (P value > 0.05). Our results shad light for a possibility of HCMV contribution in development and/or progression of this subset of breast cancers as well as could indicate for an important molecular role of BRCA1 gene defects or mutations as molecular factors in breast carcinogenesis.

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Keywords

HCMV, BRCA1, Breast tumor, ISH, IHC.

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