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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 3
First page : ( 987) Last page : ( 995)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00185.3

The Role of Sonication for the Preparation of Infection-Seeking Radiotracer Sample Versus the Standard Method

Dr Doroudi Alireza1,*, Rezaee Ehsan2, Dr Saadati Seyyed Mostafa3,**, Dr Kiasat Ali3,***, Dr Ahmadi Faramarz3,****, Etesami Behrooz4,*****, Dr Erfani Mostafa4,******

1Associate Professor, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2Candidate for Pharm D, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Ehsan8690@gmail.com

3Assistant Professor, Nuclear Medicine Department Golestan general hospital, Ahvaz Jundishapur University of Medical Sciences. Ahvaz, Iran

4Nuclear Medicine Department Golestan general hospital, Ahvaz Jundishapur University of Medical Sciences. Ahvaz, Iran

*Corresponding Author E-mail: Doroudi-a@ajums.ac.ir

**Saadati_mostafa@yahoo.com

***Kiasatali@yahoo.com

****Faramarz.ahmadi@gmail.com

*****Etesamibehrooz@gmail.com,

******msgandomkar@aeoi.org.ir

Online published on 7 July, 2018.

Abstract

The main aim of this approach is to assess the evaluation of novel green chemistry to produce the new developed [99mTc/Tricine/HYNIC] UBI 29-41radiotracer in comparison to the boiling water bath method. The 740MBq (20 mCi) of radiotracer samples were reconstituted by sonication or standard methods. The stability of radio complex samples in saline solution was investigated up 24 h post the reconstitution of cold kits. The protein bonding and partition coefficient of radiotracer samples were analyzed. The radioisotope imaging has been undertaken in order to determine the bio distribution of radiotracer samples in the infected rats with Staphylococcus aureus bacteria. The ITLC and Radio-HPLC profiles indicated that radio complex samples could be successfully reconstituted by sonication. The significant difference has not been observed in the stability, partition coefficient and protein bonding tests of radio conjugate sample which were prepared by either two methods. All infected lesions could be localized with imaging. The accumulation of radiotracer samples at the infected region versus contra lateral healthy side were identical. The outcome of this investigation indicated the reconstitution of freeze-dried UBI 29-41with 99mTc with sonication versus the boiling water bath method has the advantages like the shorter the reaction time, excellent yield, milder condition in comparison to the conventional method and environmentally benign. Green chemistry can be recommended as an alternative and reliable method for the preparation of infection-seeking radiotracer in clinical practice.

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Keywords

Green chemistry, Infection-seeking, Radioisotope imaging, Sonication, [99mTc/Tricine/HYNIC] UBI 29–41.

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