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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 3
First page : ( 899) Last page : ( 904)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00166.X

The Effect of Vitamin C towards Endothelial Dysfunction in CdCl2-induced HUVEC Culture

Sofiana Kristianningrum Dian1,2,*, Prihardina Bunga3, Khotima Husnul4, Widodo M. Aris4

1Master Program on Biomedical Science, Faculty of Medicine, Brawijaya University, Malang, Indonesia

2Department of Physiology, Faculty of Medicine, Jember University, Jember, Indonesia

3Biomedical Central Laboratory Faculty of Medicine, Brawijaya University, Malang, Indonesia

4Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia

*Corresponding Author E-mail: Kdsofiana.fk@student.ub.ac.id

Online published on 7 July, 2018.


Cardiovascular diseases are the leading cause of death globally which usually begins with endothelial dysfunction. This vascular abnormality is potentially caused by one kind of heavy metal called cadmium (Cd). Vitamin C, as an antioxidant, can serve as the free radical scavenger. This research aimed to determine the effect of vitamin C on the viability of endothelial cells and endothelial dysfunction through nitric oxide (NO) levels and malondialdehyde (MDA) in human umbilical vein endothelial cells (HUVEC) induced with CdCl2. HUVEC was induced by 24.154 μg/L CdCl2. Three treatment groups of CdCl2-induced HUVEC were employed, each given vitamin C concentrations of 50, 100, and 200 μM, respectively. Cell viability was assessed by MTT assay. Levels of NO and MDA were determined by with the calorimetric test. Cell viability was increased significantly in treatment group receiving vitamin C 100 and 200 μM. However, there were no significant differences on NO and MDA levels in vitamin C treatment groups compared to control. In conclusion, vitamin C increases endothelial cell viability and NO level, and reduces MDA levels in CdCl2-induced HUVEC. This study implies the importance of vitamin C supplementation to prevent cardiovascular diseases.



HUVEC, cadmium, vitamin C, cell viability, NO, MDA.


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