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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 2
First page : ( 657) Last page : ( 660)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00123.3

Instrumental Analysis on Optimization of Laccase Production from Pleurotus ostreatus strain PKN 04 in Different Substrate by Solid State Fermentation (SSF)

Preethi T.V.1,*, Kathireshan A.K.2, kumar G. Narendra3

1Research Scholar, Department of Microbiology, School of Life Science, Vels Institute of Science, Technology and Amp; Advanced Studies, Chennai-600 117, Tamilnadu, India

2Department of Microbiology, School of Life Science, Vels Institute of Science, Technology and Amp; Advanced Studies, Chennai-600 117, Tamilnadu, India

3Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Chennai-600 119, Tamilnadu, India

*Corresponding Author E-mail: preethynaren@gmail.com

Online published on 12 June, 2018.

Abstract

Solid state fermentation (SSF) is a green process to produce enzymes from fungi. Different substrates such as rice husk, paddy straw, sugarcane bagasse and wheat husk taken from top soil were used to produce laccase by Pleurotus ostreatus strain PKN 04. The process of SSF was characterized by FTIR. Microbial fermentation resulted in conversion of complex polymeric lignocelluloses substrates into simpler monomers. Fourier transform infrared spectroscopy (FT-IR) analyses of metabolites confirmed the breakdown and complete degradation of lignin, cellulose and polysaccharides at different fermentation time intervals of 0, 15 and 30 days.

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Keywords

Pleurotus ostreatus strain PKN 04, SSF, FT-IR.

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