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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 12
First page : ( 5273) Last page : ( 5281)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00961.7

Application of Response Surface Methodology in Process Parameter Optimization of Media for Production of Amylase

Subbaiya R.1,*, Priyanka S.2, Suresh D.3, Selvam M. Masilamani4, Balachandar R.5, Chozhavendhan S.1

1Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaga Mission's Research Foundation, Paiyanoor, Chennai-603104

2Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode-637215

3Department of Microbiology, School of Life Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai

4Department of Biotechnology, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai-600119

5Department of Biotechnology, Arulmigu Meenakshi Amman College of Engineering, Vadamavandal, Kanchipuram-604410

*Corresponding Author E-mail: ramsubbubio@gmail.com

Online published on 18 May, 2019.

Abstract

Agricultural wastes are being discarded and those wastes get accumulated in the environment. On the other hand, amylase production has become significant for its use in various industries like food, fermentation, brewing, detergent and paper industries. For this elevating demand, amylase production has to be increased. By using the agricultural waste as substrate source amylase enzyme can be produced in higher quantities. Using Bacillus subtilis as microbial source, agricultural waste can be utilised for the production of amylase in submerged fermentation as well as in solid state fermentation. Usage of synthetic media is very expensive and hence normal nutrient broth can be used. By using OVAT procedure, parameters such as temperature, pH, and substrate concentration can be varied and the optimum level at which enzyme production is higher can be determined. Response surface methodology is used for efficient production of enzyme. The enzyme produced in this process could be useful in reducing the demand of amylase in commercial use.

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Keywords

Amylase, OVAT, Submerged Fermentation, Response Surface Methodology.

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