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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 12
First page : ( 5221) Last page : ( 5226)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00952.6

Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf

Melakhessou Mohamed Akram*, Benkiki Naima, Marref Salah Eddine

Laboratoire de Biotechnologie des Molécules Bioactives et Physiopathologie Cellulaire, Faculté des Sciences de la Nature et de la vie, Département de Biologie des Organismes, Université de Batna 2, Batna, 05000, Algérie

*Corresponding Author E-mail: Akram_med@hotmail.fr

Online published on 18 May, 2019.

Abstract

Objective

The aim of the present study was to evaluate the ability of different extracts (PE, DCM, EtAOc and n-BuOH) obtained from Atractylis flava Desf to prevent oxidation using several methods in vitro.

Materials and Methods

The capacity of samples for scavenging free radicals (ABTS+, DPPH), preventing lipid peroxidation (β-carotene), chelating metal ions, CUPRAC, reducing power. The quantification of total phenols was performed using Folin-Ciocalteu reagent and the total flavonoid content was performed by the trichloroaluminum method.

Results

The highest concentrations of total polyphenols and flavonoids were found in the n-BuOH extract (122, 74 ± 0, 78 μg GAE/ml and 66, 75±6, 12 μg QE/ml, respectively). EtAOc and n-BuOH extracts were the most active for scavenging DPPH (IC50 = 158, 41±3, 24 and IC50 = 427, 53±4, 69 μg/mL, respectively), and EtAOc extract was also the best for scavenging ABTS+ radicals (IC50 = 15, 57±2, 93 μg/mL). EtAOc extract possessed the best capacity for preventing lipid peroxidation (IC50 = 16, 52±1, 87 μg/mL) and CUPRAC assay (A0.50 = 28, 58±0, 65 μg/mL). The results of ferric reducing power ability of all the tested samples were affected in a dose-dependent manner.

Conclusions

These results suggest that Atractylis flava Desf have strong antioxidant potential. Further study is necessary for isolation and characterization of t of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases.

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Keywords

Antioxidant, Atractylis flava Desf, polyphenols, flavonoids.

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