Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf
*Corresponding Author E-mail: Akram_med@hotmail.fr
The aim of the present study was to evaluate the ability of different extracts (PE, DCM, EtAOc and n-BuOH) obtained from Atractylis flava Desf to prevent oxidation using several methods in vitro.
Materials and Methods
The capacity of samples for scavenging free radicals (ABTS+, DPPH), preventing lipid peroxidation (β-carotene), chelating metal ions, CUPRAC, reducing power. The quantification of total phenols was performed using Folin-Ciocalteu reagent and the total flavonoid content was performed by the trichloroaluminum method.
The highest concentrations of total polyphenols and flavonoids were found in the n-BuOH extract (122, 74 ± 0, 78 μg GAE/ml and 66, 75±6, 12 μg QE/ml, respectively). EtAOc and n-BuOH extracts were the most active for scavenging DPPH (IC50 = 158, 41±3, 24 and IC50 = 427, 53±4, 69 μg/mL, respectively), and EtAOc extract was also the best for scavenging ABTS+ radicals (IC50 = 15, 57±2, 93 μg/mL). EtAOc extract possessed the best capacity for preventing lipid peroxidation (IC50 = 16, 52±1, 87 μg/mL) and CUPRAC assay (A0.50 = 28, 58±0, 65 μg/mL). The results of ferric reducing power ability of all the tested samples were affected in a dose-dependent manner.
These results suggest that Atractylis flava Desf have strong antioxidant potential. Further study is necessary for isolation and characterization of t of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases.
Antioxidant, Atractylis flava Desf, polyphenols, flavonoids.