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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 10
First page : ( 4699) Last page : ( 4706)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00858.2

Molecular Highlighting Analysis of Mutational CDK41, CDK6 and P27 in association with Human Mammary Tumor Virus Infection in Tissues from Iraqi Female with Ovarian Tumors

Hussein Mustafa Jaffar, Dr. Al-Alwany Shakir H. Mohammed

Biology Department, College of Science; University of Babylon

Corresponding Author E-mail:

Online published on 20 December, 2018.

Abstract

A total number of (120) formalin-fixed, paraffin embedded ovarian tissues. The mean of age of the patients with ovarian carcinoma was higher (38.98±8.763 years) than the mean age of the benign tumors group (36.28±7.825 years) and the mean age of those females in the group of healthy control (37.33±7.556years). The poorly differentiated grade (III) of ovarian cancer (OC) constituted 42.5% (17/40) whereas those with moderately(II) and well (I) differentiated grades OC constituted 30% (12/40) and 27.5% (11/40), respectively. The DNA of HMTV was detected in 40%(16/40) of tissues from ovarian cancers while HMTV DNA in the tissues from benign ovarian tumors was detected in 15% (6/40). No HMTV positive-ISH reaction was detected in healthy ovarian tissues of the control group. The positive CDK4-IHC reactions were detected in 52.5% (21 out of 40 cases) of malignant serous epithelial ovarian tumor. While, in benign ovarian tumor was expressed in 57.5%. No signal was reported in the tissues of control group. The positive CDK6-IHC reactions were disclosed in 52.5% (21/40) of serous epithelial ovarian cancer cases. Whereas, in benign ovarian tumor the positive Results was 65% (26/40) of CDK6-protein expression. No signal was reported in the tissues of control group. The positive P27-IHC reactions were detected in 62.5% (25/40) of malignant serous epithelial ovarian tumors. While, 75% (30/40) of benign ovarian tumors. No signal was reported in the tissues of control group. Our Results indicate that the HMTV might contribute to the development of subset of ovarian tumors. The present Results of the rates of defects or mutations in the CDK4; CDK6 and P27genes in relation to the grade of ovarian cancer tissues also could point for their occurrence and contribution as early events in ovarian carcinogenesis.

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Keywords

HMTV, Ovarian Cancer (OC), CDK4, CDK6, P27, ISH, IHC.

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