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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 10
First page : ( 4458) Last page : ( 4462)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00816.8

Attenuation of Cypermethrin Induced Biochemical and Histological Changes by Antioxidants in Embryonic Liver of Gallus domesticus

Sharma RK1, Kaushik D2,*, Sharma S2

1Department of Zoology, Kurukshetra University, Kurukshetra-136119

2Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119

*Corresponding Author E-mail: shivkantvats@gmail.com

Online published on 20 December, 2018.

Abstract

Cypermethrin, type-II, synthetic-cyno pyrethroid, is widely used insecticide in agricultural and pest management. Present study Aimed to investigate biochemical and histological changes induced by cypermethrin and protective effect of vitamin C in embryonic liver of Gallus domestius. Fertilized eggs were divided into four groups having 30 eggs each. Group I: served as control, Group II: as vehicle, Group III: was treated with 25 mg/ml of cypermethrin and Group IV: administered with 25 mg/ml of cypermethrin along with 100 mg of vitamin C by immersion method at embryonic day 0 for 60 minutes. Biochemical and histological changes in the developing liver were studied on embryonic day 16. Biochemical studies showed 60.6%, 49.9%, 41.3%, 22.2% decrease in Catalase, SOD, glutathione and total protein level respectively, whereas increase in the AST, ALT, ALP, and MDA level to 91%, 41.9%, 97.7%, and 75% respectively, was reported in the cypermethrin treated group as compared to the control group. In the vitamin C supplemented group, the increase in ALT, AST, ALP, MDA levels was reduced and restored level of antioxidant enzymes such as CAT, SOD and glutathione were observed. Histological studies strongly endorsed the biochemical findings. Histological changes revealed increase in hemorrhage and intracellular spaces, distorted cell membrane in the cypermethrin treated group. However diminished damage in the hepatic cells was observed in the vitamin C treated group.

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Keywords

Cypermethrin, Gallus domesticus, Hepatotoxicity, Vitamin C.

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