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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 10
First page : ( 4365) Last page : ( 4369)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00799.0

Screening of Marine Actinomycetes for Fibrinolytic Enzymes

Viswanathan K.*, Rebecca L. Jeyanthi

Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamilnadu, India

*Corresponding Author E-mail: viswanathan.bio@gmail.com

Online published on 20 December, 2018.

Abstract

Objective

This study Aims to isolate the potential fibrinolytic enzyme from actinomycetes strains.

Methods

Screening of actinomycetes from marine soil samples by using starch casein agar plate method, Protease activity by skim milk agar plate method and fibrinolytic enzyme activity by Fibrin plate method.

Results

We have isolated 114 actinomycetes strains by starch casein agar plate method and the morphology was identified by coverslip method. Out of the 114 actinomycetes strains, 40 actinomycetes strains showed protease activity by skim milk agar plate method. Protease producing actinomycetes strains were tested for fibrinolytic activity by Fibrin plate method which was indicated by growth and clear zone of fibrinolysis. Out of 40 isolates, the fibrinolytic activity was highest in 12 actinomycetes strains.

Conclusion

Based on the maximum fibrinolytic activity, BN22 was found to be a potential strain for further investigation.

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Keywords

Actinomycetes, Protease activity, Fibrinolytic activity.

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