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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 10
First page : ( 4296) Last page : ( 4302)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00786.2

Isolation and Characterization of Pigments from Microorganisms Isolated from Marine Soil

Selvi P. Senthamil1,*, Iyer Priya2

1Research Scholar, Department of Biotechnology, Women`s Christian College, Chennai-600006, India

2Associate Professor, Department of Biotechnology, Women`s Christian College, Chennai-600006, India

*Corresponding Author E-mail: brajuraj@yahoo.com, senthamil_selvi92@yahoo.com

Online published on 20 December, 2018.

Abstract

Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for applications in dairy, pharmaceutical, food etc. In this study, 3 pigments were isolated i.e. Yellow from Xanthomonas sp., Pinkish Red from Rhodotorula sp., Orange from Sarcina sp. pigmented bacterial isolates obtained from the soil, were used for pigment extraction study. To isolate and characterize the Pigment producing bacteria from marine soil. We studied that the pigment producing bacteria and to identify the colour producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacteria was used for the antimicrobial activity, antioxidant, and anticancer and transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and for dying of cloth.

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Keywords

Soil samples, GC/MS analysis, UV-Visible spectrophotometry, Carotenoidpigment extraction.

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