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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 10
First page : ( 4245) Last page : ( 4251)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00778.3

Genotypic Characterization of Staphylococcus spp. Isolated from the bodies of workers in Units of MRI, CAT, X-Ray, Restaurants and Testing Their ability to Biofilms Formation

Al-Nashe Ali Abd Raheem*, Shakir Saif Lateef**

Department of Biology, College of Education, University of Al-Qadisiyah, Iraq

*Corresponding Author E-mail: ali.alnashe@qu.eduiq

**saif.lateef@gmail.com.

Online published on 20 December, 2018.

Abstract

The present study aimed to isolating and diagnosing of Staphylococcus spp., which forms the biofilmfrom different areas of males and females workers bodies in units of: magnetic resonance imaging (MRI), computerized axial tomography (CAT) and x-ray as well as restaurants, using some phenotypic and genotypic characterization methods as well as testing their ability to phenotypically and genetically of biofilms formation. Isolates were collected within the range of hospitals in Al-Diwaniyah Governorate at the period from 1/11/2016 till 1/4/2017 and a total of 200 samples distributed equally on predefined isolation units. The results showed that superior the bacterial isolation percent of male workers in these units exceeded the females at all sources of isolation. Staphylococcus isolates were diagnosed by using the API-20 Staph systemand the species of genus (Staph. epidermidis, Staph. haemolyticus and Staph. aureus) have been affiliated a higher frequency of isolates number (57 isolates), which were divided between 23, 21 and 13 isolates, respectively. The isolates consisted of biofilm formation were 13 isolates from Staph. epidermids, 16 isolates from Staph. haemolyticus and 10 isolates of Staph. aureus. Genetic diagnosis of all isolates was done by using the PCR technique in terms of having a 16S rRNA gene as well as investigating the ability of Staphylococcusisolates to biofilms formation in terms of having the icaA gene. The results of phenotypic investigation were identical to the results of genetic investigation.

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Keywords

Genotypic characterization, Staphylococcus, Biofilms formations.

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