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Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 1
First page : ( 275) Last page : ( 280)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00051.3

Polymorphism of IL-4 (−590) and IL-6 (−174) is not associated with Chronic Periodontitis in Babylonian Population

Abd Frial Gemeel*

Iraq, University of Babylon, Department of Biology

*Corresponding Author E-mail: frialabd@yahoo.com

Online published on 12 June, 2018.

Abstract

Aim

Study was aimed to find the relationship between chronic periodontitis with polymorphism of IL-4 and IL6 genes and their concentration in saliva and serum.

Material and method

the study was included patients with chronic periodontitis (60 subjects) and healthy control (40 subjects), bacteria associated with both subjects have been isolated. The genetic variation in the sequence of the IL-4 and IL-6 has been detected by single strand conformation polymorphism (SSCP) technique. the level of IL-4 and IL-6 in saliva and serum was measured by enzyme linked immunosorbent assay (ELISA).

Results

The total viable count of bacteria that collect from patients with chronic periodontitis and healthy control showed that There was significant differences (increase in the number of bacteria) between study groups in the aerobic bacteria, the number of bacteria in healthy groups was 3.1×103±39.15 CFU/ml, and in patient was 2.4×105 ±65.93 CFU/ml P<0.001 and the anaerobic bacteria in healthy and patient was 2.3×102±22.61 CFU/ml and 1.2×104±45.95 CFU/ml respectively (P≤ 0.05). The most common bacteria isolated in this study were P. Porphyromonasgingivalis, P. Prevotellaintermedia, Tannerellaforsythensis, Peptostreptococcus spp., Aggregatibacter actinomycetemcomitans, Streptococcus spp. And Staphylococcus aureus. SSCP technique showed that there weren't any sequence variation between patients with periodontitis and healthy control subjects in the sequence of IL-4 and IL-6 genes in Babylonian population.

Conclusion

Polymorphism of IL-4 and IL-6 is not associated with chronic periodontitis in Babylonian population.

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Keywords

Musculoskeletal symptoms, Ambulance workers, helmets, fatigue, stress of neck and back.

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