(3.236.51.151)
Users online: 2198    [ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2018, Volume : 11, Issue : 1
First page : ( 38) Last page : ( 40)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2018.00007.0

Isolation and Screening the Pharmacological activities of Vegetative and Spore-crystal Proteins from Bacillus thuringiensis

Kavitha R.*, Damodharan N., Sangeetha S., Remya P. N., Saraswathi T. S.

Department of Pharmaceutics, SRM College of Pharmacy, SRM University, Kattankulathur, Tamil Nadu, India

*Corresponding Author E-mail: kavithar2007@gmail.com

Online published on 12 June, 2018.

Abstract

Bacillus thuringiensis, the most successful and most widely used microbial insecticide, produces crystal proteins. The physiological significance of the crystal proteins poorly understood except for the potent insecticidal activity. In the current study, an attempt made to isolate vegetative and spore-crystal proteins from Bacillus thuringiensis NCIM2514 and to perform their hemolytic and antioxidant activities. The proteins were separated by salting out methods then by using SDS-PAGE. The molecular protein weight of the vegetative and sporecrystal protein identified as the presence of low molecular weight protein. Pharmacological activities as antioxidant and hemolytic studies were performed for the crude proteins and found as Spore-crystal proteins contain more anti-oxidant when compared to vegetative proteins.

Top

Keywords

Bacillus thuringiensis (Bt), spore-crystal proteins, vegetative proteins. SDS-PAGE, Antioxidant.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
518,969,358 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.