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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 7
First page : ( 2249) Last page : ( 2254)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00398.5

Development and Validation of a Bioanalytical method for the Determination of Levamisole Residue in Backyard Poultry Egg

M Hossain d Kamal1,*, Nahar Kamrun1, M Mazumder ohammed Ehsanul Hoque1, M Wahiduzzaman d1, Alqahtani Ali S.2, Dr Gestier Tony1, Hamid Kaiser1,*

1Vetafarm Pty Ltd. R and D Centre, Wagga Wagga, NSW, 2650, Australia.

2Faculty of Pharmacy, King Saud University, Saudi Arabia

*Corresponding author e-mail: kamal@vetafarm.com.au

Online published on 17 October, 2017.

Abstract

Development of a bioanalytical method is often tricky and labour intensive due to its nature or the criteria set by regulatory bodies. In the present study, a simple, reliable and sensitive analytical method was developed for the determination of levamisole residue in chicken eggs. Levamisole and ronidazole (internal standard, IS) were separated on a C18 reversed-phase HPLC column. Following liquid-liquid extraction, chromatographic separation was accomplished with a mobile phase consisting of acetonitrile: 0.05M KH2PO4 at ratio of 20: 80, and the drug was detected at 235nm using a UV detector at a flow rate of 1.0ml/min at the ambient temperature. Linearity was obtained over the range 0.3125–10.0μg/ml for levamisole hydrochloride with lower limit of quantitation of 0.3125μg/ml. For each level of quality control samples, inter-and intra-day precision (%CV) was within ±15%. Absolutes extraction recovery of drug from plasma was ≥;60%. The residue limit of levamisole gradually increased from day 1 to day 3 and then declined from day4 to day 7. The highest level of residue after oral administration of 80mg/kg levamisole to chickens was on day 3 (0.599μg/g). This level was lower than the recommended maximum residue limit (MRL), thus, no withholding period was required for egg consumption following the administration of the levamisole solution.

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Keywords

Levamisole, Bioanalytical, Residue, Withholding period, Backyard poultry.

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