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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 7
First page : ( 2213) Last page : ( 2220)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00391.2

Simultaneous estimation of Rosiglitazone Maleate, Glimepride and Amlodipine Besylate in Tablet Dosage formulation by RP-HPLC

Singh Rajeev K.1,*, Sharma Pushpendra2

1Research Scholar, Department of Chemistry, Sri Satya Sai University of Technology and Medical Sciences, Opp. Oil-fed Plant, Bhopal-Indore Road, Sehore, Madhya Pradesh-466001, India.

2Sri Satya Sai University of Technology and Medical Sciences, Opp. Oil-fed Plant, Bhopal-Indore Road, Sehore, Madhya Pradesh-466001, India.

*Corresponding Author E-mail: rajeevbiochem@gmail.com

Online published on 17 October, 2017.

Abstract

A simple, rapid and precise reversed-phase high performance liquid chromatography method for simultaneous estimation of rosiglitazone maleate, glimepride and amlodipine besylate in a marketed tablet dosage form has been developed and validated. At optimum condition was performed on a C18 column by ratio of mobile phase Methanol: Water: Ortho Phosphoric Acid (75: 25: 0.2) with maintain of pH 4.5±0.01 at 1.0 mL/min. Selected wavelength at 230 nm and sample volume injected 20 μL at ambient temperature. Total run time was 10 min; rosiglitazone maleate, glimepride and amlodipine besylate were eluted with retention time of 1.75, 2.22 and 6.483 respectively. The developed method was validated as per ICH guideline. Validation revealed that method is specific, rapid, accurate, precise, reliable and reproducible.

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Keywords

RP-HPLC, Method Development, Anti-diabetic, Validation.

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