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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 7
First page : ( 2047) Last page : ( 2052)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00357.2

A Simple Protocol for the Isolation of Quercetin from Trigonella foenumgraecum L. Leaves and Antioxidant analysis for Combating Toxicity

Durga M1,*, Nathiya S2, Devasena T3

1Department of Biochemistry and Bioinformatics, Dr. MGR Janaki College of Arts and Science, Chennai, Tamilnadu, India

2Department of Nanotechnology, Bharath Institute of Medical Sciences, Chennai

3Centre for Nanoscience and Technology, Anna University, Chennai-600025, India

*Corresponding Author E-mail: durga_2k7@yahoo.co.in

Online published on 17 October, 2017.

Abstract

Trigonella foenum-graecum L. is commonly known as fenugreek. It is an annual herb, commonly used in many parts of India. In the present study total flavonoid estimation, antioxidant activity and isolation of quercetin were carried out. Sequential extraction was performed using chloroform, ethyl acetate and methanol. Highest yield was observed in methanol extract (10.04%). Among the three solvents ethyl acetate (3.2mg/g) showed maximum amount of total flavonoid content followed by chloroform (3.05mg/g) and methanol (1.0mg/g) extracts. Antioxidant potential was evaluated by DPPH free radical scavenging assay and Super oxide scavenging assay for ethyl acetate and chloroform extracts. Among the extracts ethyl acetate extract showed maximum antioxidant potential at 67.45% and 48.565% for DPPH free radical scavenging assay and Super oxide scavenging assay respectively. The isolation of quercetin was performed in ethyl acetate extract and validated using HPLC by comparing with known Standard quercetin.

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Keywords

Flavonoid, Fenugreek, DPPH, SO, HPLC.

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