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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 6
First page : ( 1924) Last page : ( 1928)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00337.7

Production, optimization, Characterization and Immobilization of Glucose oxidase from Aspergillus species

Jagathy K.1, Kalpana K.1, Rajeshkumar S.2,*

1Department of Microbiology, Sri Akilandeswari Women's College, Wandiwash-604 408, TN, India

2School of Bio-Sciences and Technology (SBST), Vellore Institute of Technology University, Vellore-632014, TN, India

*Corresponding Author E-mail: ssrajeshkumar@hotmail.com

Online published on 22 September, 2017.

Abstract

Aspergillus sp was isolated from soil and screened for glucose oxidase production. In this present investigation isolates 7 fungal isolates from different soil samples has shown positive glucose oxidase activity. The various parameters like Carbon Source (g/l) (glucose, sucrose, fructose, maltose) nitrogen Source (g/l) (peptone, yeast extract, beef extract), calcium carbonate (g/mL), pH, Temperature (°C), incubation time and agitation rate used for the maximum enzyme production. The highest GOx production was obtained in glucose (100 g/L) followed by sucrose. Peptone (3–4 g/L) was found to be effective nitrogen source for GOx production when compared to other nitrogen sources. CaCO3-40 g/mL, pH-6 and temperature 35°C and and incubation time 70 h for growth and production of glucose oxidase from screened culture of Aspergillus sps.

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Keywords

Glucose oxidase, Aspergillus sp, Optimization.

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