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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 6
First page : ( 1635) Last page : ( 1640)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00287.6

Antimicrobial activity of silver nanoparticles synthesized from Ficus benghalensis against human pathogens

Sudhakar Thukkaram1,*, P Balashanmugam2, Premkumar Jayapal1, A Anisha1, D Karthika1, Sapkota Roshan1, Rijal Sakar1

1Department of Biomedical Engineering, School of Bio and Chemical Engineering, Sathyabama University, Chennai-119

2CHORD Division, CSIR, CLRI, Chennai

*Corresponding Author E-mail: drsudhakar35@gmail.com

Online published on 22 September, 2017.

Abstract

In the context of present world Nanotechnology has found it's way in every important aspects of our daily lives from diagnostic techniques, drug delivery, sunscreens, antimicrobial bandages to disinfectants. In this study the synthesis of nanoparticles was done by reducing silver nitrate using the bacteria Bacillus species. Formation of nanoparticles was confirmed by UV-visible spectroscopy with maximum absorbance at 434nm, characteristic of silver nanoparticles by using various analytical studies. FTIR is taken to confirm the chemical bonding present in AgNPs. XRD and FE-SEM was used to determine the metallic characteristics of AgNPs as well as size and morphology of AgNPs. The size of AgNPs is 41.13nm. Disc diffusion method was done for antimicrobial activity, further comparative analysis and synergistic activity was determined. Comparative analysis of synthesized AgNPs was done with water, AgNO3, extracted samples and AgNPs. Further synergetic activity was done to confirm the antimicrobial activity of AgNPs. AgNPs shows good antimicrobial activity against human pathogens so it can be used in drug delivery system.

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Keywords

AgNPS - Silver nanoparticles, XRD-X-Ray Diffraction, FE-SEM-Field Emission Scanning Electron Microscope.

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