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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 4
First page : ( 1115) Last page : ( 1119)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00201.3

Statistical Evaluation of Medium Components by Plackett-Burman method for Laccase from Pseudomonas aeruginosa ADN04 using Submerged Fermentation

Arunkumar T.1,*, Narendrakumar G2, Anand D. Alex3

1Department of Bioinformatics, Sathyabama University, Chennai-600119

2Department of Biotechnology, Sathyabama University, Chennai-600119

3Department of Biomedical Engineering, Sathyabama University, Chennai-600119

*Corresponding Author E-mail: t.arunkumar.t@gmail.com

Online published on 17 July, 2017.

Abstract

The effect of medium components on laccase production from using Pseudomonas aeruginosa ADN04 was studied. Placket Burman design was used to evaluate the significant parameters that have large effect on the fermentation and with the experimental design a successful result was obtained. K2HPO4, NaNO3, CaCl2, NH4Cl, CuSO4, NH4H2PO4, ZnSO4, Glucose, Sucrose, Yeast Extract, MgSO4, KH2PO4 was screened for the better media composition. Sucrose, K2HPO4, NH4Cl, Yeast Extract, CaCl2, were standardized according to the F values.

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Keywords

Plackett-Burman Design (PBD), Laccase, submerged fermentation.

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