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Research Journal of Pharmacy and Technology
Year : 2017, Volume : 10, Issue : 11
First page : ( 3990) Last page : ( 3996)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.5958/0974-360X.2017.00724.7

RP-HPLC Method for Simultaneous Estimation of Cilnidipine and Chlorthalidone

Pawar Vijaykumar T.1,*, Pawar Shubhangi V.2, More Harinath N.1, Kulkarni Anita S.3, Gaikwad Dinanath T.4

1Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, Maharashtra, India

2Department of Pharmaceutical Chemistry, Shri. Santkrupa College of Pharmacy, Ghogaon

3Department of Pharmaceutical Chemistry, Government College of Pharmacy, Karad

4Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Kolhapur-416013, Maharashtra, India

*Corresponding Author E-mail: vijaydash1982@rediffmail.com

Online published on 26 March, 2018.

Abstract

Aim of present study was development of RP-HPLC method for simultaneous estimation of cilnidipine and chlorthalidone. RP-HPLC method for simultaneous estimation of Cilnidipine and Chlorthalidone was carried out by using methanol: water (80: 20 v/v) at a flow rate of 1 ml min-1 with UV detection at 231.6 nm. Both drugs showed linearity in the concentration range of 10–70 μg ml-1.for CIL and 10–70 μg ml-1.for CHLT. When marketed pharmaceutical preparations were analyzed, the result obtained by the proposed method was found in good agreement with labeled claims. Recoveries in all these method were in the range of 99.60–100.18%. Results obtained were statistically validated and were found to be reproducible. These methods can be applied for routine analysis of formulations containing these drugs. For analytical methods validation parameters such as linearity, precision, accuracy, specificity, limit of detection, limit of quantization and robustness were determined according to ICH Q2B guidelines.

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Keywords

Cilnidipine, Chlorthalidone, RP-HPLC, ICH Q2B, Validation.

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