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Research Journal of Pharmacy and Technology
Year : 2008, Volume : 1, Issue : 4
First page : ( 395) Last page : ( 397)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.

RP-HPLC Method Development and Validation for Assay of Levetiracetam in Tablet Dosage Form

Valarmathy J1,*, Samueljoshua L2, Rathinavel G1, Thanuja C Selvin1, Sivakumar T1

1Department of Pharmacy, Nandha College of Pharmacy, Erode-638052, Tamilnadu, India

2Department of Pharmacy, Padmavathi College of Pharmacy, Dharmapuri, Tamilnadu, India

*Corresponding Author E-mail: valarmathyjoshua@rediffmail.com

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for estimation of Levetiracetam in its tablet dosage form. Separation was done by using mobile phase consists of buffer solution (pH 2.8) and acetonitrile in the ratio of 90:10. Chromatography separations were carried out on prontosil C18 column (150X4.6mn; 5μm) at a flow rate of 1.2 ml/min and UV detection at 215nm and the retention time for levetiracetam is 4minutes. The linear dynamic response was found to be in the concentration of 45μg-270μg/ml. The slope, intercept and correlation coefficient were found to be 0.9999 respectively. The percentage recovery of Levetiracetam was found to be 99.08%. Proposed methods were found to be simple, accurate, precise and rapid and could be used for routine analysis. This condition is applied only for tablet dosage form. The statistical parameters and recovery studies were carried out and reported.

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Keywords

Levetiracetam, Estimation, Tablets, RP-HPLC.

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