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Research Journal of Pharmacology and Pharmacodynamics
Year : 2017, Volume : 9, Issue : 2
First page : ( 46) Last page : ( 56)
Print ISSN : 0975-4407. Online ISSN : 2321-5836.
Article DOI : 10.5958/2321-5836.2017.00009.X

Anti-arthritic activity of Annona squamosa leaves methanolic extract on adjuvant induced arthritis in rats

Kumar A. Suneel1,*, Venkatarathanamma V.2, Saibabu V. Naga3, Tentu Nageswara Rao4, Kota Pavan Kumar5, Kumar K. Suneel6, Vijayalakshmi A.7

1Department o Biochemistry, Acharya Nagarjuna University, Nagarjunanagar, Andhra Pradesh, India.

2Department of Zoology, Acharya Nagarjuna University, Nagarjunanagar, A.P., India

3Department o Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, Andhra Pradesh, India.

4Department o Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.

5Department of pharmacy, SRM University, Chennai, Tamilnadu, India.

6Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India.

7Department o Marine Living Resources, Andhra University, Visakhapatnam, Andhra Pradesh, India.

*Corresponding Author E-mail: tnraochemistry@gmail.com

Online published on 22 September, 2017.


The current research has aimed to evaluate the anti-arthritic effect of Annona squamosamethanolic leaves extract(ASME) on the progression of adjuvant induced arthritis in Wistar rats. ASME was obtained by bioactivity guided fraction by 5-Lipooxygenase inhibitory activity. The ASME was administrated orally at 100 and 200 mg/kg body weight for 35 days to the experimental animals. Arthritis was induced on day 8 by a single intra-plantar injection of 0.1 ml suspension of heat killed Mycobacterium tuberculosis (100 µg/animal) in incomplete Freund's adjuvant of left foot pads of female Wistar rats. The anti-arthritic activity extract was screened by measuring hind limb paw volume, biochemical and haematological parameters, pathological and radiography changes. The levels of pro-inflammatory cytokines TNF-α, IL-1β and inflammatory mediators PGE2, LTB4 was measured in serum samples on day 35. The stabilizing ability of lipid peroxide and activities of enzymatic antioxidants catalase, superoxide dismutase (SOD) and non-enzymatic antioxidants reduced glutathione (GSH)levels were measured in liver. Treated groups ASME 100 and 200 mg/kg and prednisolone 10 mg/kg significantly decreased hind paw volume, diminished serum levels of TNF-α, IL-1β, PGE2 and LTB4, reduced MDA levels and increased levels of catalase, SOD and GSH levels in liver and promising results of serum biochemistry, haematology, histopathology and radiographic changes suggests the administration of ASME was effective in modulating the inflammatory response and conquer the advancement of arthritis in experimental animal model. The safety of ASME was established (LD50>2000 mg/kg) by acute oral toxicity limit test according to OECD guideline 423. These findings may help to improve the treatment of rheumatoid arthritis.



Rheumatoid arthritis, Annona squamosaleaves methanolic extract (ASME), Inflammation, 5-Lipooxygenase, TNF-α, IL-1β, PGE2 and LTB4.


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