In Vitro Anti Cancer Activity of Leaf Extracts of Azima tetracantha Lam against Tryphan Blue Exclusion Method by using Dal Cell Lines
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Traditional medicine has a long history of serving people all over the world. In recent years, the use of traditional medicine information in cancer research received considerable interest. Ethanolic Extract of Azima tetracantha (lam) has been used in traditional and folklore medicine for the treatment of cancer. The aim of the present study was to evaluate the effect of Chloroform extract, Ethyl acetate extract, Ethanolic Extract and Aqueous extract of Azima tetracantha (lam) against trypan blue dye exclusion assay against Daltons ascites lymphoma cell lines.
Materials and methods
The tumor cells were aspirated from the peritoneal cavity of tumor bearing mice were washed thrice normal saline and checked for viability using trypan blue dye exclusion method. The cell suspension (1x106cells in 0.1ml) was added to tubes containing 10, 20, 50, 100 and 200 concentration of the test compounds and the volume was made up to 1ml using phosphate buffered saline (PBS). Control tube contained only cell suspension. These assay mixture were incubated for 3 hour at 37°C.
The number dead cells were found to increase with an increase in the concentration of drug used. The percentage toxicity was found to increase indicate that the given drug has potent cytotoxic activity in-vitro condition. For chloroform extract, the trypan exclusion method is evaluated as 2, 8, 14, 34 and 52% of dead cells. Ethyl acetate extract, the trypan exclusion method is evaluated as 0, 4, 11, 22 and 31% of dead cells. The ethanol extract, the trypan exclusion method is evaluated as 8, 14, 20, 40 and 60% of dead cells. For aqueous extract, the trypan exclusion method is evaluated as 4, 11, 22, 36 and 52% of dead cells.
All these findings enable to conclude that the dose of Ethanolic Extract of Azima tetracantha (lam) possess a protective effect against DAL cell lines.
Dalton's Ascitic Lymphoma, Chloroform extract, Ethyl acetate extract, Ethanolic Extract and Aqueous extract of Azima tetracantha (lam), Trypan blue exclusion assay.