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Research Journal of Pharmacognosy and Phytochemistry
Year : 2017, Volume : 9, Issue : 1
First page : ( 17) Last page : ( 22)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.5958/0975-4385.2017.00003.6

In-vitro cytotoxic activity of plant saponin extracts on breast cancer cell-line

Soni Anjali*, Femida Patel, Sharma Preeti

Department of Biotechnology, Veer Narmad South Gujarat University, Udhana-magdalla Road, Surat, 395007, Gujarat, India

*Corresponding Author E-mail: anjalisoni@vnsgu.ac.in

Online published on 26 April, 2017.

Abstract

The aim of present work is to study anticancer and antioxidant activity of saponins present in Guar (Cyamopsis tetragonoloba) and Soybean (Glycine max) seeds. Seeds were finely powdered and methanolic extracts were prepared by Soxhlet method to study the antioxidant activity. Saponin extracts were prepared to study their effect on cancer cell line. The antioxidant activity was determined by ABTS radical scavenging assay and anticancer activity by MTT and SRB methods on MCF-7 breast cancer cell-line. Maximum 55.29 at 200 ug/ml and 65.18 at 100 ug/ml percentage inhibitions of ABTS radical was obtained by Guar and Soybean seed methanolic extracts respectively. The IC50 value of Guar saponin extract on MCF-7 cell line was found to be 12.4 and 13.75 μg/ml and Soybean saponin extract 6.875 and 7.5 μg/ml using MTT and SRB methods respectively. Result suggests that both plants methanolic extract possess appreciable antioxidant activities. Soybean saponin seed extracts exhibit anticancer activity at very low concentration which can be investigated further to determine its potential use in cancer therapy.

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Keywords

Cytotoxicity, DPPH, MCF-7, Saponins, MTT.

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