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Research Journal of Pharmacognosy and Phytochemistry
Year : 2020, Volume : 12, Issue : 1
First page : ( 19) Last page : ( 23)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.5958/0975-4385.2020.00004.7

Expression, Purification and Bioassay of Cry55Aa protein against tomato root knot Nematode, Meloidogyne incognita

Manivannan A.1, Kumar K. K.3, Varanavasiappan S.3, Manimegalai S.1, Poornima K.2, Devrajan B. C.2, Sudhakar D.3, Balasubramani V.1,*

1Department of Agricultural Entomology, Tamil Nadu Agricultural University (TNAU), Coimbatore-641003

2Department of Nematology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University (TNAU), Coimbatore-641003

3Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University (TNAU), Coimbatore-641003

*Corresponding Author E-mail: balasubramani.v@gmail.com

Abstract

A nematicidal Cry55Aa protein was expressed and purified from E. coli cells. Higher level of expression was observed in culture induced with 0.1mM of IPTG after four hours of induction. The Cry55Aa protein was purified by resuspending the crude protein in solubilizing buffer at pH 12 for 120 min. When Meloidogyne incognita was tested against different concentration of purified protein, a clear dose response lethality was observed with an LC50 value of 31.4μg/ml.

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Keywords

Cry55Aa protein, expression, purification, bioassay, M. incognita., .

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