Phytochemical and Physico-chemical characterization with Amino acid profile of Nypa fruticans, Wurmb. from Kerala
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The aim of the study was to analyse the physico-chemical characters, mineral composition, phytochemical and amino acids of leaf and stem of Nypa fruticans, Wurmb. Moisture content, ash content, acid insoluble ash, water soluble ash, extractive value in water and alcohol and mineral composition were determined in physico-chemical analysis. In mineral analysis, macronutrients such as N, P and K and micronutrients like Fe, Cu, Zn and Mn were analysed. Mineral analysis revealed that stem of N. fruticans, Wurmb. contained more K (6.21mg/g) than leaves. Potassium obtained in higher amount than Nitrogen and Phosphorous. Fe and Zn were recorded high in the stem. Analysis of amino acids and mineral nutrients could reveal the nutrient and therapeutic potential of the plant. Both primary and secondary metabolites were estimated from the leaf and stem of N. fruticans, Wurmb. Carbohydrates, proteins and lipids were the primary metabolites and phenol, flavonoids, tannins, alkaloids, sterols, terpenoids, coumarins and saponins were the secondary metabolites. Carbohydrates and proteins observed high in the stem of Nypa compared to leaves. Among the secondary metabolites, phenols, flavonoids and alkaloids were found more than the other secondary metabolites. Amino acid content of leaf and stem of N. fruticans, Wurmb. were determined using Pico-Tag method. Amino acids were confirmed in the sample by comparing with the retention time of standard amino acids. Among the 17 amino acids, 16 amino acids were identified in all the samples, except cysteine. Total quantity of amino acids was 15.94 mg/g in the leaves and 21.95 mg/g in the stem. Non essential amino acid alanine obtained in highest quantity (4.89 mg/g) in the stem.
Nypa fruticans, Wurmb, HPLC, Minerals, Amino acids, Phytochemicals.