Screening of Garcinia cambogia for in-Vitro Anti-Cancerous Activity against Colon Adenocarcinoma (Caco-2) Cell Line
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Objective: Garcinia cambogia, commonly known as ‘malabar tamarind’ is considered to have great medicinal properties. The dried fruit rind of Garcinia cambogia was used in this present study to evaluate its anti cancerous potential against Caco-2 cell lines. Materials and Methods: In vitro cytotoxicity of fruit rind extracts of Garcinia cambogia in methanol, distilled water, ethyl acetate, hexane and chloroform were evaluated by MTS ((3-(4, 5dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), Phenazinemethosulfate (PMS)) assay against Caco-2 (human colorectal cancer) cell lines. Cell cycle analysis and apoptosis assay were done by using 2% paraformaldehyde, 1X PBS (Phosphate Buffered Saline Ph=7), DAPI (4′, 6-Diamidine-2′-phenylindole dihydrochloride), FITC-Annexin V and Propidium Iodide. Conclusion: The results suggest that different extracts of fruit rind of Garcinia cambogia (Methanol, Distilled water, Ethyl acetate, Hexane, Chloroform) was found to have insignificant anticancer activity at concentration of 1000μg/ml, 100μg/ml, 10μg/ml against Caco-2 cell lines.
Garcinia cambogia, anti-cancer, in vitro, MTS assay, Apoptotic assay, Cell Cycle Analysis.