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Research Journal of Pharmacognosy and Phytochemistry
Year : 2018, Volume : 10, Issue : 4
First page : ( 272) Last page : ( 276)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.5958/0975-4385.2018.00043.2

Screening of Garcinia cambogia for in-Vitro Anti-Cancerous Activity against Colon Adenocarcinoma (Caco-2) Cell Line

Banu Sadika*, Ramakrishnaiah T. N

Department of Biotechnology and Applied Genetics, Ramaiah College of Arts, Science and Commerce. Bangalore, India

*Corresponding Author E-mail: sadiqarajeeb.83@gmail.com

Online published on 27 December, 2018.

Abstract

Objective: Garcinia cambogia, commonly known as ‘malabar tamarind’ is considered to have great medicinal properties. The dried fruit rind of Garcinia cambogia was used in this present study to evaluate its anti cancerous potential against Caco-2 cell lines. Materials and Methods: In vitro cytotoxicity of fruit rind extracts of Garcinia cambogia in methanol, distilled water, ethyl acetate, hexane and chloroform were evaluated by MTS ((3-(4, 5dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium), Phenazinemethosulfate (PMS)) assay against Caco-2 (human colorectal cancer) cell lines. Cell cycle analysis and apoptosis assay were done by using 2% paraformaldehyde, 1X PBS (Phosphate Buffered Saline Ph=7), DAPI (4′, 6-Diamidine-2′-phenylindole dihydrochloride), FITC-Annexin V and Propidium Iodide. Conclusion: The results suggest that different extracts of fruit rind of Garcinia cambogia (Methanol, Distilled water, Ethyl acetate, Hexane, Chloroform) was found to have insignificant anticancer activity at concentration of 1000μg/ml, 100μg/ml, 10μg/ml against Caco-2 cell lines.

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Keywords

Garcinia cambogia, anti-cancer, in vitro, MTS assay, Apoptotic assay, Cell Cycle Analysis.

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