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Research Journal of Pharmacognosy and Phytochemistry
Year : 2018, Volume : 10, Issue : 2
First page : ( 145) Last page : ( 151)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.
Article DOI : 10.5958/0975-4385.2018.00021.3

Preliminary pytochemical screening and HPTLC finger printing of Clerodendrum inerme (L.) Gaertn.

Basavaraj Hurakadli Swathi1,*, Hebbar S. Chaithra2, Ravikrishna S.3, Prabhu Suchitra Narayan4

1PG Scholar, Department of Dravyaguna, SDM College of Ayurveda, Udupi

2Associate Professor, Department of Dravyaguna, SDM College of Ayurveda, Udupi

3Associate Professor, Department of Agadatantra, SDM College of Ayurveda, Udupi

4Research Officer, S.D.M Centre for Research in Ayurveda and Allied Sciences, Udupi, Karnataka

*Corresponding Author E-mail: swaguun@gmail.com

Online published on 7 July, 2018.

Abstract

Objective

The present work was carried out to perform the powder microscopy, HPTLC finger printing and preliminary phytochemical evaluation of whole plant of Clerodendrum inerme (L.) Gaertn.

Method

The study was carried out in terms of powder microscopy, HPTLC fingerprinting was done using CAMAG HPTLC system equipped with Linomat 5 applicator, TLC scanner 4 and WIN CATS-4 software were used and preliminary phytochemical parameters were analysed.

Results

Powder microscopic features shows presence of epidermal cells containing chlorophyll and stomata, parenchyama cells of abundant fragments of outer layer of roots in association with exodermis, presence of vessels, parenchyama cells with starch, bundle of fibres interspersed with starch grains in a surrounding are seen. Preliminary phytochemical analysis showed the presence of alkaloid, steroid, carbohydrate, tannin, coumarins, phenols and resin. And HPTLC documentation revealed presence of many phytochemicals with different Rf values and HPTLC densitometric scan of the plates showed numerous bands and peaks.

Conclusion

Various characters that were observed in this study will help in identification of C. inerme (L.) Gaertn. in crude form. It can be concluded that HPTLC fingerprint analysis of whole plant extract of C. inerme (L.) Gaertn. can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.

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Keywords

Clerodendrum inerme (L) Gaertn, Kshudra Agnimantha, Whole plant, Powder microscopy, Preliminary phytochemical analysis, HPTLC fingerprinting.

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