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Research Journal of Pharmacognosy and Phytochemistry
Year : 2009, Volume : 1, Issue : 1
First page : ( 30) Last page : ( 34)
Print ISSN : 0975-2331. Online ISSN : 0975-4385.

Optimization of process parameters for the production of L-asparaginase from an isolated fungus

Sreenivasulu V1,*, Jayaveera KN2, Rao P Mallikarjuna3

1Department of Biotechnology, KLR Pharmacy College, Paloncha-507115, Khammam (District), A.P, India

2Oil Technological Research Institute, JNT University, Anantapur–15001, A.P, India

3International Medical University, Kualalumpur, Malaysia-57000

*Corresponding Author: V. Sreenivasulu Asst. Professor, Dept. of Biotechnology, KLR Pharmacy College, Paloncha507115,Khammam-District, A.P, India. Mobile no: 094413 22168, E maiI: cnuvurathi@yahoo.com, cnuvurathi@rediffmail.com

Online published on 22 March, 2013.

Abstract

The extracellular L-asparaginase production by fungi isolated from soil samples by using pH and dye based method. Various physical and chemical parameters were optimized under submerged fermentation for Lasparaginase production. Maximum productivity of L-asparaginase (19.5 U/ml) was achieved by employing medium containing 2% (w/v) Lasparagine as substrate concentration, 1%(w/v) glucose as carbon source, 1% (w/v) ammonium sulphate as an additional nitrogen source with the incubation period of 96 h and incubation temperature at 30°C, initial pH 6.5 at an inoculum level 20% (v/v) with 48 h old inoculum was found to be optimum for maximum yield.

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Keywords

L-asparaginase, optimization, submerged fermentation.

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