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RESEARCH JOURNAL OF PHARMACEUTICAL DOSAGE FORMS AND TECHNOLOGY
Year : 2020, Volume : 12, Issue : 4
First page : ( 298) Last page : ( 300)
Print ISSN : 0975-234X. Online ISSN : 0975-4377.
Article DOI : 10.5958/0975-4377.2020.00049.X

Anticancer activity of Psidium guajava leaf extracts on breast cancer cell line

Yadav Akshay R.*, Mohite Shrinivas K.

Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Sangli, Maharashtra, India-415404.

*Corresponding Author E-mail: akshayyadav24197@gmail.com

Online published on 10 February, 2021.

Abstract

The present study was aimed to evaluate the anticancer activity of various leaf extracts of Psidiumguajava against the breast cancer cell line (MCF-7). Trypan Blue dye exclusion test is used to determine the number of viable cells present in a cell suspension. The theory is based on the assumption that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not.If the cell suspension is simply mixed with the dye and then visually checked to see if the cells are taking or lacking dye. An effective cell would have a clear cytoplasm and a blue cytoplasm would have a nonviable cell. Periodic tests of cell viability provide an early measure of fresh cell consistency prior freezing. Excellent viability beyond or equal to 95%. Results of the study showed a high potential for anticancer activity in the human cancer cell line MCF-7 in different leaf extracts of Psidiumguajava. Further detailed investigation of the plant active components for an accurate mechanism of action will make a significant contribution to the development of new pharmaceuticals.

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Keywords

Psidiumguajava, Breast cancer, Trypan blue assay, Cell viability.

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