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Research Journal of Pharmaceutical Dosage Forms and Technology
Year : 2020, Volume : 12, Issue : 2
First page : ( 89) Last page : ( 92)
Print ISSN : 0975-234X. Online ISSN : 0975-4377.
Article DOI : 10.5958/0975-4377.2020.00016.6

Development of an Analytical Method to Determine Extracts of Different Species of Glycyrrhizin by HPTLC

Salunke Chetan*, Chittam Kailaspati, Pawar Amol, Chaudhari Harshal

Department of Pharmacognosy, Annasaheb Ramesh Ajmera College of Pharmacy Nagao Dhule Dist: Dhule 424002, Maharashtra, India

*Corresponding Author E-mail: amolraje957@gmail.com

Online published on 10 August, 2020.

Abstract

A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of glycyrrhizin has been developed and validated for the determination of glycyrrhizin in herbal extracts. The analyte was extracted with ethanol and applied on TLC aluminium plates along with standard using Linomat 5 spray on sample applicator (CAMAG). Analysis of glycyrrhizin was performed on pre-coated TLC aluminium plates with silica gel as the stationary phase and prewashed with methanol. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of ethyl acetate-methanol-water-formic acid (15:2:1:1 v/v/v/v). Spectrodensitometric scanning was performed by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 252nm. The system was found to give compact spots for glycyrrhizin (Rf value of 0.34 ± 0.04). The linear regression analysis data for the calibration plots showed good linear relationship with r2=0.9981 in the concentration range 2–15 μl with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines the method was validated for precision, recovery, stability in solution and robustness. The glycyrrhizin content quantified from herbal extracts and from the formulation was found well within limits. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of glycyrrhizin.

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Keywords

Glycyrrhiza glabra, Glycyrrhizin, HPTLC, Method development and validation, Monoammonium Glycyrrhizinate (MAG), Gel.

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