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ORYZA- An International Journal on Rice
Year : 2016, Volume : 53, Issue : 2
First page : ( 126) Last page : ( 135)
Print ISSN : 0474-7615. Online ISSN : 2249-5266.

Isolation and characterization of the promoter of RGG1 using Agrobacterium-mediated transient assay

Swain Durga Madhab1,4, Sahoo Ranjan Kumar1, Tripathy Baishnab Charan2,4, Tuteja Narendra1,3,*

1International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi-110067, India

2Jawaharlal Nehru University, New Delhi-110067, India

3Amity University, Noida-201313, India

4Ravenshaw university, Cuttack, Odisha-753003

*Corresponding author e-mail: narendra@icgeb.res.in; ntuteja@amity.edu

Gene Bank Accession Number of RGG1: GU111573.1

Online published on 8 February, 2017.

Abstract

The heterotrimeric G protein complex, comprising of G α, G β, and G γ subunits, is an evolutionarily conservedsignaling molecular machine which transmits signals from transmembrane receptors to downstream target proteins. Now-a-days their functions in plant stress-signalling have been reported. Here we have isolated the promoter of rice G-protein γ subunit 1(RGG1) from rice genomic DNA. In silico analysis revealed that promoter of RGG1 contained a TATA, a CAAT motif and also harbors some important stress and hormone associated cis regulatory elements, including E-box, GAAAAA, GATA-box, CACATG, CCGTCC and CCACC. Functional analyses were performed by Agrobacterium-mediated transient assay in tobacco leaves. Very high level of GUS activity was observed in agro infiltrated tobacco leaves by the construct carrying the RGG1 promoter:: GUS, subjected to abiotic stress and exogenous hormonal treatments. Stress-inducible nature of RGG1 promoter opens possibility for the study of the gene regulation under stress condition. Therefore, the result may be useful in the field of agriculture and biotechnology

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Keywords

Cis acting elements, RGG1 promoter, Place database, Agroinfiltration, GUS activity.

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