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Journal of Plant Disease Sciences
Year : 2017, Volume : 12, Issue : 1
First page : ( 29) Last page : ( 36)
Print ISSN : 0973-7456. Online ISSN : 0976-2388.

Bioefficacy of Psoralea corylifolia L. seeds against Sclerotium rolfsii, incitant of collar rot of chickpea

Shitole A. V.*, Gade R. M., Wavare S. H.

Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444104 (M.S.) India

*Email: amolshitole1690@gmail.com

Online published on 30 September, 2019.

Abstract

Laboratory bioassays were carried out to evaluate the antifungal potential of acetone, chloroform, distilled water, ethanol and methanol extract of Psoralea corylifolia L. seeds against Sclerotium rolfsii causing collar rot of chickpea (Cicer arietinum L.). Different concentrations of solvent extracts (125, 250, 375 and 500 μl) were prepared and their effect against S. rolfsii were tested by using poisoned food technique. Maximum mycelial growth inhibition of the fungal pathogens was observed in methanol extract at 500 μl concentration (98.23%). Methanolic extract was fractioned on thin layer chromatography (TLC) plates using different eluting solvent systems. From all solvent systems, toluene: ethyl acetate: methanol (24: 04: 02 v/v) separated more number of secondary metabolites from methanol extract of P. corylifolia seeds. In TLC-bioautography none of the separated compound showed prominent activity against the tested fungal pathogen. Antifungal activity of methanol extract P. corylifolia seeds clearly indicated that the inhibition recorded in poison food technique was due to the synergetic effect of most of the compounds from the extract. The present study concludes that methanol extract of P. corylifolia seeds possess antifungal activity and can be used for management of S. rolfsii.

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Keywords

Psoralea corylifoliaS. rolfsii, bioefficacy.

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