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Journal of Immunology and Immunopathology
Year : 2004, Volume : 6, Issue : supp1
First page : ( 98) Last page : ( 99)
Print ISSN : 0972-0561.

Chlorpyrifos induced apoptosis in avian lymphocytes

Ravindra P.V.*, Singhal L.K., Ambwani S, Gupta M, Chauhan R.S.

Department of Pathology, College of Veterinary and Animal Sciences, GB Pant University of Agriculture and Technology, Pantnagar-263 145, (Uttaranchal).

*E-mail: raviravindra1@rediffmail.com

Abstract

A study was conducted to explore the in vitro immunotoxic effects of chlorpyrifos- an organophosphate insecticide on avian lymphocytes. Lymphocytes separated from spleen of birds were treated with NOEL/103, NOEL/104 and NOEL/105 dose of chlorpyrifos. DNA was then isolated and examined using agarose gel electrophoresis for ladder pattern. Results revealed a clear ladder pattern of DNA fragmentation in comparison to controls, which is a hall mark of apoptosis. Therefore, it is concluded that the chlorpyrifos executes immunotoxicity by inducing apoptotic death in lymphocytes.

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Key words

Chlorpyrifos, Avian lymphocytes, Apoptosis, DNA ladder pattern.

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