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Journal of Immunology and Immunopathology
Year : 2009, Volume : 11, Issue : 2
First page : ( 33) Last page : ( 38)
Print ISSN : 0972-0561. Online ISSN : 0973-9149.

Immune response of broiler chicks to different Ranikhet disease vaccines using live RDF and killed R2B strain in oil adjuvant

Basu S, Singh S D*, Dhama K, Bhuvaneswari T

Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, (Uttar Pradesh), 243122, INDIA

*Corresponding Author: E-mail: sdsingh2005@rediffmail.com

Abstract

A study was carried out to assess the immune response of broiler-chickens to different Ranikhet disease (RD) vaccines viz., live RDF, live RDF in-oil and live RDF + killed RB-in-oil. A dose of 106..2 EID/0.1 ml was used for oculonasal instillation of live RDF vaccine, while a same dose of live RDF vaccine was mixed thoroughly with oiladjuvant (Lanolin-liquid paraffin) at 1:2 ratio and 0.3 ml of vaccine inoculated through sub-cutaneous route. For live RDF+ killed R2B-in-oil vaccine, same dose of live RDF vaccine instilled oculonasally along with 0.3 ml of formalin-inactivated, oil-adjuvanted RB vaccine with 108.4 EID/0.1 ml inoculated subcutaneously. The humoral antibody levels as detected by HI and ELISA appeared significantly higher in live RDF-in-oil and live RDF + killed R2B-in-oil groups at different weeks post-vaccination (wpv) compared to group with live RDF vaccine administered alone. The cell-mediated immune (CMI) response, studied by lymphocyte proliferation assay, revealed that the mean stimulation indices (SI) of peripheral blood lymphocytes (PBLs) to non-specific Con-A stimulation and specific RDV stimulation in all the vaccinated groups appeared significantly higher than control group on 2nd, 3rd, 4th and 5th wpv thus suggesting better efficacy of oil-adjuvanted vaccines to enhance the CMI responses in chickens. Challenge study revealed that the live RDF vaccine protected the birds completely up to 4th week of age but not till marketing age (6–8 weeks), while oil-adjuvanted vaccines offered a complete protection against virulent RD virus till 8th week of age and also revealed refractivity of birds to multiplication of virus. In an overall assessment, live RDF-in-oil as well as live RDF + killed R2B-in-oil vaccines appeared superior to induce a high and long-lasting immunity in broiler-chickens against RDV infection following vaccination at 1st week of age.

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Keywords

Ranikhet disease, Broiler chicks, ELISA, Haemagglutination-inhibtion (HI) test, Humoral immunity, Oil adjuvant.

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