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Journal of Immunology and Immunopathology
Year : 2009, Volume : 11, Issue : 1
First page : ( 48) Last page : ( 52)
Print ISSN : 0972-0561. Online ISSN : 0973-9149.

In vitro antiviral activity of plant extracts against Infectious Bursal Disease Virus

Virmani Meenakshi1,2, Kapoor S.1,3, Garg S L.1,4, Virmani N.1,5

1Department of Veterinary Biochemistry, College of Veterinary Sciences, CCS Haryana Agricultural University, Hisar, 125 001, (Haryana).

2Department of Veterinary Physiology, College of Veterinary Sciences, GADVASU, Ludhiana, (Punjab).

3Department of Veterinary Microbiology, National Research Centre on Equines, Sirsa Road, Hisar, (Haryana), INDIA.

4Department of Veterinary Biochemistry, National Research Centre on Equines, Sirsa Road, Hisar, (Haryana), INDIA.

5National Research Centre on Equines, Sirsa Road, Hisar, (Haryana), INDIA.


The present study was conducted with the aim to evaluate the activity of extracts of medicinal plants (Moringa oleifera, Holarrhena antidysenterica, Synzium aromaticum, Allium sativum, Piper nigrum and Azadirachta indica) against Infectious Bursal Disease Virus (IBD virus) in in vitro primary Chicken Embryo Fibroblast (CEF) cell culture. For this, aqueous and alcoholic extracts of leaves/fruit/seed/stem of these medicinal plants were prepared using Soxhlet apparatus. The extracts were tested for cytotoxicity in CEF cell culture and the non-cytotoxic concentration of extracts was used for evaluation of antiviral activity by cytopathic effect inhibition assay. The antiviral activity of the extracts was calculated using Reed and Muench formula. The alcoholic extract of clove of A. sativum, seed of A. indica and aqueous extract of P. nigrum seeds and leaves were found to possess antiviral activity. Extracts of other plants showed no antiviral activity against IBD virus.


Key words

Allium sativum, Antiviral activity, Azadirachta indicaHolarrhena antidysenterica, Infectious Bursal Disease Virus, medicinal plants, Moringa oleiferaPiper nigrumSynzium aromaticum.


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