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Journal of Immunology and Immunopathology
Year : 2009, Volume : 11, Issue : 1
First page : ( 36) Last page : ( 41)
Print ISSN : 0972-0561. Online ISSN : 0973-9149.

Development of IL-2, IL-18 and IFN-γ gene based genetic adjuvants for use along with CSFV DNA vaccine in pigs

Ratta Barkha2,1, Nautiyal Binita3,1Reader, Ravindra P V.4,1, Chaturvedi Uttara1, Palia Sudesh1, Subudhi P K.1, Kumar Rajiv1, Tiwari Sangeeta3,1Reader, Tiwari A K.1,*

1Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122 (Uttar Pradesh), INDIA.

2Immunochemistry Laboratory, Department of Biochemistry, Indian Veterinary Research Institute, Izatnagar-243122 (Uttar Pradesh), INDIA.

3Department of Animal Science, M J P Rohilkhand University, Bareilly, 243006 (Uttar Pradesh).

4Department of Cardio-thoracic Surgery, Biological Science Division, University of Chicago, Chicago, Illinois-60637, U S A.

*E-mail: aktiwari63@yahoo.com

Abbreviations used

CSFV

=

(Classical swine fever virus),

CSF

=

(Classical swine fever),

IFN-γ

=

(Interferon gamma),

IL2

=

(Interleukin-2),

IL-18

=

(Interleukin-18),

RT-PCR

=

(Reverse transcriptase-Polymerase chain reaction).

Abstract

The cytokine genes when administered along with DNA vaccine may work as immunological adjuvant and enhance immune responses. Hence, the study was undertaken to clone and characterize the pig IFN-γ, IL-2 and IL-18 cytokine genes. The pig IFN-γ, IL-2 and IL-18 gene were amplified using specific primers by RT-PCR and cloned into pTZ57R/T cloning vector. The clone was confirmed by digestion analysis using KpnI and ApaI restriction enzymes. Further, the gene fragment was sub-cloned into eukaryotic expression vector pcDNA 3.1(+) and confirmed by restriction digestion as described above. These recombinant plasmids can be used as genetic adjuvants to DNA vaccine against CSFV.

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Key words

RT-PCR, adjuvants, cytokines.

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