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Journal of Immunology and Immunopathology
Year : 2008, Volume : 10, Issue : 1
First page : ( 65) Last page : ( 69)
Print ISSN : 0972-0561.

Modulation of immune responses by aqueous extract of Argemone Mexicana leaves

Goel A*, Kumar D, Bhatia AK**

Department of Microbiology & Immunology, College of Veterinary Sciences, Uttar Pradesh Pandit Deen Dayal Upadhyay Veterinary University, Mathura (U.P.) India.

*E-mail: anjanagoel2000@yahoo.com

**E-mail: akbhatia33@yahoo.com

Abstract

Nontoxic dose of Argemone mexicana aqueous leaves extract was determined in albino rats as well as in rabbits by giving different quantities (ranging from 50 mg/kg to 500 mg/kg body weight) orally for 20 days. The 250 mg/kg body weight of A. mexicana aqueous extract of leaves was used as an ideal and safe nontoxic dose. Immunomodulatory effect of filtered aqueous extract of A. mexicana leaves was studied in albino rats. A group of 4 albino rats were fed orally with 250 mg/kg body weight/day for 23 days. Cell mediated immune response was measured by total lymphocyte count, E-rosette formation and Di-nitro chloro benzene (DNCB) sensitized hypersensitivity test. An 18% decrease in rosette forming cell in A. mexicana aqueous leaves extract fed rats was noted. Percentage of lymphocyte separated on Histopaque 1077 from peripheral blood was also decreased to the level of 26% showed lymphocytopenia. DNCB hypersensitivity test showed 5.26% increase in thickness at 24 hours where as control showed 47.36% increase in thickness. Comparing with control, inhibition of 88.8% and 83.3% was calculated at 24 and 48 hours, respectively. These studies showed the T-cell suppressive effect of aqueous extract of A. mexicana leaves. Humoral immunoregulatory profile to antigenic challenge of Salmonella typhimurium by quantitating the agglutinating antibodies by tube agglutination test indicated a significant increase in antibody titer in the serum 1:1280 in comparison to control stating 1:320. These studies show that A. mexicana leaves extract is a humoral immune response enhancer and cell mediated immunosuppressive agent. This might be beneficial in autoimmune diseases related to cell mediated immunity and organ transplantation.

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Key words

Argemone mexicana, Humoral immunity, Cell mediated immunity, Immunomodulation.

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