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Journal of Immunology and Immunopathology
Year : 2008, Volume : 10, Issue : 1
First page : ( 55) Last page : ( 59)
Print ISSN : 0972-0561.

Haemagglutination inhibition assay for Equine Chorionic Gonadotropin (ECG) detection in pregnant mare serum

Virmani Meenakshi1,*, Gupta AK1, Virmani Nitin1, Garg SK2

1National Research Centre on Equines. Sirsa Road, Hisar-125001 (Haryana).

2Department of Veterinary Physiology, College of Veterinary Sciences, CCS HAU, Hisar-125 001, (Haryana) India.

*E-mail: virmanim2003@yahoo.com, Present address- Department of Veterinary Physiology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana (Punjab).

Abstract

A haemagglutination inhibition assay for measuring eCG (Equine Chorionic Gonadotrophin) in pregnant mare serum has been standardized to detect pregnancy in mares at an early stage. A polyclonal antiserum against eCG was raised in rabbit. The eCG was detected in the serum at 45 days of gestation which rose sharply to reach maximum level around 82 days of gestation and declined afterwards. The results were found to be more accurate and precise than to those obtained by bioassay in immature female mice by injecting serum from pregnant mares and calculating ovarian weight gain.

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Key words

haemagglutination inhibition, Equine Chorionic Gonadotrophin, pregnancy detection.

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