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The Journal of Indian Botanical Society
Year : 2018, Volume : 97, Issue : 3and4
First page : ( 38) Last page : ( 44)
Print ISSN : 0019-4468. Online ISSN : 2455-7218.
Article DOI : 10.5958/2455-7218.2018.00014.1

Qualitative and quantitative analysis of thaumatin like proteins (TLPS) in In Vivo and In Vitro cultures of gymnema sylvestre

Zimare Saurabha B1,2, Javir Gitanjali3, Otageri Jyoti3, Pai Kalpana3, Malpathak Nutan P1,*

1Department of Botany, Savitribai Phule Pune University, Pune-411007, (MH), India

2Naoroji Godrej Centre for Plant Research (NGCPR). C/o Lawkim Motors Division. Gate no. 431, Shindewadi, Post Shirwal, Dist. Satara. Maharashtra, India

3Department of Zoology, Savitribai Phule Pune University, Pune-411007, (MH), India

*Email: mpathak@unipune.ac.in

Online published on 19 February, 2019.


Plant produces different pathogenesis related proteins as a defence mechanism against pathogenic conditions and in adaptation to stressful environment. TLPs are a group of proteins from PR 5 category which have shown homology with thaumatin from Thaumatococcus daniellii and have shown antibacterial activity. Exploration of PR proteins from in vivo plant materials (leaves) and from in vitro (cell suspension and shoot) cultures of G. sylvestre using Western blot and ELISA techniques has been reported for the first time. Western blot analysis showed a prominent band at 22 kDa which correspond to the TLPs of Gymnema sylvestre which compared with standard TLPs. ELISA analysis showed higher quantity of TLPs (2.067 μg/3g) in in vivo plant materials as compared to shoot cultures (1.729 μg/3g) and cell suspension cultures (1.721 μg/3g).



ELISA, G. sylvestre, SDS-PAGE, TLPs, Western blot.


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