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Journal of Hill Agriculture
Year : 2013, Volume : 4, Issue : 2
First page : ( 74) Last page : ( 77)
Print ISSN : 0976-7606. Online ISSN : 2230-7338.

In vitro regeneration using nodal sections and shoot tips of gypsophila cv. ‘Bristol Fairy’

Thakur Shailja1,*, Dhiman SR2, Sharma YD2, Singh AK1

1Centre for Protected Cultivation Technology, Indian Agricultural Research Institute, New Delhi, 110 012, India

2Department of Floriculture and Landscaping, Dr YS Parmar University of Horticulture and Forestry, Nauni Solan, Himachal Pradesh– 173 230, India

*E mail: thakurshailja10@gmail.com

Online published on 21 August, 2014.

Abstract

Adventitious shoots were successfully regenerated from shoot tip and nodal section explants of Gypsophila paniculata L. The efficiency of shoot regeneration for cv. ‘Bristol Fairy’ was tested on 12 media based on Murashige and Skoog basal medium containing different concenterations of BAP, kinetin and 2ip or their combinations. Highest efficiency of adventitious shoot regeneration was obtained with 6μM BAP when shoot tip explant was used. For in vitro rooting different combination of auxins were used in MS medium out of which 5μM NAA + 5μM IBA was found to be best with 100% rooting of shoots.

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Keywords

Adventitious shoots, Gypsophila, shoot tip and nodal section explant, regeneration.

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