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Journal of Food Legumes
Year : 2011, Volume : 24, Issue : 4
First page : ( 313) Last page : ( 316)
Print ISSN : 0970-6380. Online ISSN : 0976-2434.

Genetic diversity in Helicoverpa armigera (Hübner) from different agroclimatic zones of India using RAPD markers

Deepa M., Srivastava C.P.

Department of Entomology and Agricultural Zoology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221 005, India; Email: deepa.icfre@gmail.com

Online published on 14 December, 2011.

Received:  9  March,  2011; Accepted:  23  September,  2011.

Abstract

Genetic diversity of Helicoverpa armigera (Hübner) populations collected from different agro-climatic zones of India was determined by RAPD markers. The DNA extracted from larvae were subjected to polymerase chain reaction using 25 RAPD primers. The highest number of 30 amplicon levels was produced by the primer OPA 16, 29 levels by OPA 01, 22 levels by OPA 05 and 21 levels by OPD 07. The lowest of 8 amplicon level was noticed with OPD 13. On an average there were 14.84 amplicon levels per primer, of which 14.76 were polymorphic, indicating high variability among H. armigera populations. Molecular data analysis clustered the H. armigera populations in to two groups. The first group (X) comprised 8 populations which is furthe subdivided into X1 and X2 at a similarity coefficient of 0.13 while the distinct second group (Y) consisted of only Dharwad population. Genetic similarities among geographical populations from the data were within the similarity co- efficient ranging from 0.15 to 0.40. Using the unweighted pair group method analysis, H. armigera collected and reared in Varanasi stood out as unique in one cluster while the insects collected and reared in other states grouped separately.

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Keywords

Agro-climatic zones, Genetic diversity, Helicoverpa armigera, RAPD markers.

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