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Journal of Food Legumes
Year : 2009, Volume : 22, Issue : 1
First page : ( 11) Last page : ( 17)
Print ISSN : 0970-6380.

Cross-species amplification of microsatellite loci and diversity analyses in blackgram

Souframanien J.*, Gopalakrishna T.

Nuclear Agriculture and Biotechnology Division, Mumbai, 400 085, India.

*E-mail: souf@scientist.com

Abstract

A set of 72 microsatellite primers developed from cowpea (Vigna unguiculata (L.) Walp), mungbean (Vigna radiata (L.) Wilczek), azuki bean (Vigna angularis (Wild) Ohwi and Ohashi), common bean (Phaseolus vulgaris L) was tested for their ability to amplify microsatellite loci in black gram. Forty nine (68.0%) microsatellite primer pairs showed cross species amplification. Out of the 49 transferable markers, 42 (85.7%) primer pairs showed polymorphism among the 29 black gram genotypes used in the study. The 49 microsatellite primer pairs amplified 93 alleles out of which 83 were polymorphic with an average of 1.7 polymorphic alleles per primer pair. The polymorphic information content (PIC) value of microsatellite markers ranged from 0 to 0.98 with an average of 0.48. No significant correlation was detected between repeat number and the allele number (r =−0.260) or PIC (r =0.013). A dendrogram constructed based on microsatellite markers grouped the 29 black gram genotypes into 5 main clusters. The Jaccard's similarity coefficient ranged from 0.35 to 0.81. Genotype specific microsatellite markers were observed in the study. The finding suggests that cross amplification of microsatellite markers from closely related species will help in the development of locus specific microsatellite marker in Vigna mungo for which sequence information is not yet available.

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Key words

Cross species amplification, Genetic diversity, Microsatellite polymorphism, Vigna mungo L.

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