Use of genomic resources in improvement of Vigna species
|Dr. Subhojit Datta, Senior Scientist and In-Charge of the Biotechnology Unit at Indian Institute of Pulses Research, Kanpur has over nine years research experience in the area of plant genomics and pulses biotechnology. After completing his Masters’ and Doctorate degree in Biotechnology from NRC on Plant Biotechnology,IARI, New Delhi, he joined ARS in 2000. He has also worked at CAES Genomics Facility, University of California, Davis, USA in 2004 and 2008. Dr. Datta has over 15 research articles in the reputed journals and eight book chapters to his credit. He is serving several international journals like Plant Breeding, Plant Genetic Resources: Characterization and Utilization, Canadian Journal of Microbiology, Journal of Plant Biochemistry & Plant Biotechnology, Journal of Food Legumes as Subject Editor or reviewer. Dr. Datta has won several awards-ISCA Young Scientist Award-2002 by Indian Science Congress Association, India; BOYSCAST Fellowship-2008 of Department of Science and Technology, India to Visit University of California, Davis, USA and Fellowship of Indian Society of Pulses Research and Development-2009.|
|Dr. Sanjeev Gupta, Principal Scientist at Indian Institute of Pulses Research, Kanpur is an accomplished Vigna breeder with more than 19 years experience on basic and applied research. He obtained his M.Sc. (Ag) and Ph.D. degree from CSAUA&T, Kanpur. Before joining at IIPR, Kanpur in 1999, Dr. Gupta had worked on biotechnology and breeding programmes of fodder grasses and legumes at Indian Grassland and Fodder Research Institute, Jhansi for 10 years. He has been involved in development of two varieties of rajmash, one each of mungbean and urdbean and two varieties of perennial grasses. He has also developed a tissue culture protocol of grass species-Dichanlhiwn annulalum. Dr. Gupta has identified several useful donors in urdbean, mungbean and rajmash for different traits. He has special interest in molecular markers and attended International Course on Genetic Distinction and Molecular Markers from International Agricultural Centre, Wageningen, the Netherlands. He is serving several international and national journals as Subject Editor or reviewer. He is Secretary of Indian Society of Pulses Research and Development and Fellow of Range Management Society of India. He has organized International Conference and National Symposium on Legumes as Organizing Secretary. He has to his credit more than 70 publications including research papers, book chapters, books, bulletins, manuals, popular and technical articles. He is the recipient of presitigious ICAR Award-Dr. Rajendra Prasad Award for book ‘Dalhan’.|
The Vigna is one of the most important genus in the Fabaceae. Among many species of this genus, nine are domesticated as food crops. The average yields of these crops are very low due to inherently low yield potential of the present cultivars and their susceptibility to diseases like Mungbean Yellow Mosaic Virus (MYMV), powdery mildew and cercospora leaf spots. Various genomic resources have been dev.eloped for mungbean, urdbean, azuki bean and cowpea for accelerating the breeding efforts in these crops. The utility of marker assisted selections in improvement of Vigna species is limited largely due to limited marker polymorphism within the species. Efforts are being made to develop high-throughput markers with greater resolution. Different types of molecular markers such as RAPDs, RFLPs, AFLPs and SSRs have been developed and mapped onto chromosomes. These markers have been used for identification and mapping of genes and QTLs for important traits like seed weight, resistance against MYMV and powdery mildew and tolerance against bruchids. Eleven genetic linkage maps were developed so far but very few contained enough markers to condense into putative 11 linkage groups. Comparative genome mapping between mungbean and several other legumes including azuki bean, common bean, cowpea and lablab bean revealed various levels of macrosynteny. Comparison between urdbean and azuki bean maps revealed high degree of genomic colinearity. Modest beginning has been made in developing RAC libraries to facilitate map-based cloning of genes and QTLs. Advances in developing genomic resources such as linkage maps, high-throughput markers and RAC libraries will be helpful to accelerate genetic improvement of Vigna species.